Dave Ross gave a presentation on profiling PHP and JavaScript performance. He recommended using the Xdebug profiler for PHP, which provides profiling information that can be viewed in tools like Webgrind. For JavaScript profiling, he suggested using the Firebug console to turn profiling on and off and view profiling results within the Firebug interface. The goal of profiling is to identify sections of code to optimize for better speed or lower memory usage.